Elliott Stieglitz
Assistant Professor
+1 415 502-2649
+1 415 502-4372

Biography

Juvenile myelomonocytic leukemia (JMML) is a blood cancer that affects young children and is often difficult to diagnose and treat. Currently available therapies cure only half of patients, with some children experiencing an aggressive disease course while a rare group get better with very little treatment. We have now shown that presence of secondary mutations via deep sequencing or droplet digital PCR predicts a poor outcome. We have most recently show that DNA methylation profiling can predict both good and poor responders at diagnosis.

We are also running a clinical trial through the Children's Oncology Group for patients with relapsed and refractory JMML. This trial will test the safety and efficacy of the oral MEK inhibitor, trametinib. This trial is based on extensive pre-clinical testing and a strong genomic rationale. This trial marks an important step towards more effectively treating patients with Ras driven leukemia using targeted agents.

Education and Training

, 2014 Pediatric Hematology Oncology Fellowship, University of California, San Francisco
, 2011 Pediatric Residency, New York Presbyterian - Weill Cornell
MD, 2008 School of Medicine, Stony Brook

Publications

Fabrizio VA, Kernan NA, Boulad F, Cancio M, Allen J, Higman M, Margossian SP, Mauguen A, Prockop S, Scaradavou A, Shah N, Spitzer B, Stieglitz E, Yeager N, O'Reilly RJ, Brentjens RJ, Jan Boelens J, Curran KJ. Low toxicity and favorable overall survival in relapsed/refractory B-ALL following CAR T cells and CD34-selected T-cell depleted allogeneic hematopoietic cell transplant. Bone Marrow Transplant. 2020 May 10.
Dueck ME, Lin R, Zayac A, Gallagher S, Chao AK, Jiang L, Datwani SS, Hung P, Stieglitz E. Precision cancer monitoring using a novel, fully integrated, microfluidic array partitioning digital PCR platform. Sci Rep. 2019 12 20; 9(1):19606.
Friend BD, Bailey-Olson M, Melton A, Shimano KA, Kharbanda S, Higham C, Winestone LE, Huang J, Stieglitz E, Dvorak CC. The impact of total body irradiation-based regimens on outcomes in children and young adults with acute lymphoblastic leukemia undergoing allogeneic hematopoietic stem cell transplantation. Pediatr Blood Cancer. 2020 02; 67(2):e28079.
Julia Meyer, Maximillian Schönung, Christian Flotho, Adam B. Olshen, Mark Hartmann, Christoph Plass, Yusuke Okuno, Yoshiyuki Takahashi, Norihiro Murakami, Manabu Wakamatsu, Mignon L. Loh, Charlotte M. Niemeyer, Hideki Muramatsu, Daniel B. Lipka, Elliot Stieglitz. DNA Methylation As a Biomarker of Outcome in JMML: An International Effort Towards Clinical Implementation. Blood. 2019 Nov 13; 134(Supplement_1):1693-1693.
Matthew Nix, Yu-Hsiu T. Lin, Huimin Geng, Makeba Marcoulis, Paul Phojanakong, Donghui Wang, Veronica Steri, Jeffrey Whitman, Sagar Bapat, Elliot Stieglitz, Anne Deucher, Kristie L White, Byron C Hann, Arun P. Wiita. In Vitro-Selected Nanobody-Based Cellular Therapy Targeting CD72 for Treatment of Refractory B-Cell Malignancies. Blood. 2019 Nov 13; 134(Supplement_1):1337-1337.
Chao AK, Meyer JA, Lee AG, Hecht A, Tarver T, Van Ziffle J, Koegel AK, Golden C, Braun BS, Sweet-Cordero EA, Smith CC, Dvorak CC, Loh ML, Stieglitz E. Fusion driven JMML: a novel CCDC88C-FLT3 fusion responsive to sorafenib identified by RNA sequencing. Leukemia. 2020 Feb; 34(2):662-666.
Hecht A, Meyer J, Chehab FF, White KL, Magruder K, Dvorak CC, Loh ML, Stieglitz E. Molecular assessment of pretransplant chemotherapy in the treatment of juvenile myelomonocytic leukemia. Pediatr Blood Cancer. 2019 11; 66(11):e27948.
de Smith AJ, Lavoie G, Walsh KM, Aujla S, Evans E, Hansen HM, Smirnov I, Kang AY, Zenker M, Ceremsak JJ, Stieglitz E, Muskens IS, Roberts W, McKean-Cowdin R, Metayer C, Roux PP, Wiemels JL. Predisposing germline mutations in high hyperdiploid acute lymphoblastic leukemia in children. Genes Chromosomes Cancer. 2019 10; 58(10):723-730.
Capri Y, Flex E, Krumbach OHF, Carpentieri G, Cecchetti S, Lißewski C, Rezaei Adariani S, Schanze D, Brinkmann J, Piard J, Pantaleoni F, Lepri FR, Goh ES, Chong K, Stieglitz E, Meyer J, Kuechler A, Bramswig NC, Sacharow S, Strullu M, Vial Y, Vignal C, Kensah G, Cuturilo G, Kazemein Jasemi NS, Dvorsky R, Monaghan KG, Vincent LM, Cavé H, Verloes A, Ahmadian MR, Tartaglia M, Zenker M. Activating Mutations of RRAS2 Are a Rare Cause of Noonan Syndrome. Am J Hum Genet. 2019 06 06; 104(6):1223-1232.
Iacobucci I, Wen J, Meggendorfer M, Choi JK, Shi L, Pounds SB, Carmichael CL, Masih KE, Morris SM, Lindsley RC, Janke LJ, Alexander TB, Song G, Qu C, Li Y, Payne-Turner D, Tomizawa D, Kiyokawa N, Valentine M, Valentine V, Basso G, Locatelli F, Enemark EJ, Kham SKY, Yeoh AEJ, Ma X, Zhou X, Sioson E, Rusch M, Ries RE, Stieglitz E, Hunger SP, Wei AH, To LB, Lewis ID, D'Andrea RJ, Kile BT, Brown AL, Scott HS, Hahn CN, Marlton P, Pei D, Cheng C, Loh ML, Ebert BL, Meshinchi S, Haferlach T, Mullighan CG. Genomic subtyping and therapeutic targeting of acute erythroleukemia. Nat Genet. 2019 04; 51(4):694-704.
Maximilian Schoenung, Elliot Stieglitz, Hideki Muramatsu, Norihiro Murakami, Mark Hartmann, Manuel Wiesenfarth, Yusuke Okuno, Adam B. Olshen, Christoph Plass, Mignon L. Loh, Charlotte Niemeyer, Christian Flotho, Daniel B. Lipka. DNA Methylation Subgroups in Juvenile Myelomonocytic Leukemia: An International Collaborative Analysis and Development of a Common Diagnostic Platform. Blood. 2018 Nov 29; 132(Supplement 1):3093-3093.
Adam J de Smith, Geneviève Lavoie, Kyle M Walsh, Sumeet Aujla, Erica Evans, Helen M Hansen, Ivan Smirnov, Alice Y Kang, Martin Zenker, John J Ceremsak, Elliot Stieglitz, Roberta McKean-Cowdin, Catherine Metayer, Philippe P Roux, Joseph L Wiemels. Germline GAB2 Mutations in Childhood Acute Lymphoblastic Leukemia. Blood. 2018 Nov 29; 132(Supplement 1):388-388.
Andrew E. Place, Traci M. Blonquist, Elliot Stieglitz, Todd M Cooper, Lia Gore, Richard Aplenc, Mignon L. Loh, Melinda Pauly, Rachel E. Rau, Michael J. Burke, Julia Etchin, A. Thomas Look, Maria Luisa Sulis, Lewis B. Silverman. Phase I Study of the Selinexor in Relapsed/Refractory Childhood Acute Leukemia. Blood. 2018 Nov 29; 132(Supplement 1):1405-1405.
Kong G, You X, Wen Z, Chang YI, Qian S, Ranheim EA, Letson C, Zhang X, Zhou Y, Liu Y, Rajagopalan A, Zhang J, Stieglitz E, Loh M, Hofmann I, Yang D, Zhong X, Padron E, Zhou L, Pear WS, Zhang J. Downregulating Notch counteracts KrasG12D-induced ERK activation and oxidative phosphorylation in myeloproliferative neoplasm. Leukemia. 2019 03; 33(3):671-685.
Stieglitz E, Dinh T, Phelps AS, Pampaloni MH, Olshen AB, Robbins E. ABVD Without Radiation for Newly Diagnosed Pediatric and Young Adult Patients With Hodgkin Lymphoma: A Single Center Retrospective Analysis of 28 Consecutive Patients. J Pediatr Hematol Oncol. 2018 05; 40(4):290-294.
Dvorak CC, Satwani P, Stieglitz E, Cairo MS, Dang H, Pei Q, Gao Y, Wall D, Mazor T, Olshen AB, Parker JS, Kahwash S, Hirsch B, Raimondi S, Patel N, Skeens M, Cooper T, Mehta PA, Grupp SA, Loh ML. Disease burden and conditioning regimens in ASCT1221, a randomized phase II trial in children with juvenile myelomonocytic leukemia: A Children's Oncology Group study. Pediatr Blood Cancer. 2018 07; 65(7):e27034.
Stieglitz E, Mazor T, Olshen AB, Geng H, Gelston LC, Akutagawa J, Lipka DB, Plass C, Flotho C, Chehab FF, Braun BS, Costello JF, Loh ML. Genome-wide DNA methylation is predictive of outcome in juvenile myelomonocytic leukemia. Nat Commun. 2017 12 19; 8(1):2127.
Alan Cantor, Elisa Dorantes-Costa, Hui Huang, Sara Garcia, Elliot Stieglitz, Mignon Loh, Guo-Cheng Yuan. Dysregulation of the transcription factor runx1 in juvenile myelomonocytic leukemia. Experimental Hematology. 2017 Sep 1; 53:s51.
Yoshimi A, Balasis ME, Vedder A, Feldman K, Ma Y, Zhang H, Lee SC, Letson C, Niyongere S, Lu SX, Ball M, Taylor J, Zhang Q, Zhao Y, Youssef S, Chung YR, Zhang XJ, Durham BH, Yang W, List AF, Loh ML, Klimek V, Berger MF, Stieglitz E, Padron E, Abdel-Wahab O. Robust patient-derived xenografts of MDS/MPN overlap syndromes capture the unique characteristics of CMML and JMML. Blood. 2017 07 27; 130(4):397-407.
Whale AS, Devonshire AS, Karlin-Neumann G, Regan J, Javier L, Cowen S, Fernandez-Gonzalez A, Jones GM, Redshaw N, Beck J, Berger AW, Combaret V, Dahl Kjersgaard N, Davis L, Fina F, Forshew T, Fredslund Andersen R, Galbiati S, González Hernández Á, Haynes CA, Janku F, Lacave R, Lee J, Mistry V, Pender A, Pradines A, Proudhon C, Saal LH, Stieglitz E, Ulrich B, Foy CA, Parkes H, Tzonev S, Huggett JF. International Interlaboratory Digital PCR Study Demonstrating High Reproducibility for the Measurement of a Rare Sequence Variant. Anal Chem. 2017 02 07; 89(3):1724-1733.
Sandrine Niyongere, Nolwenn Lucas, Samir Sansil, Christopher L Cubitt, Maria E. Balasis, Jodi Kroeger, Qing Zhang, John J Ceremsak, Elliot Stieglitz, YuLong Zhao, Markus Ball, Rami S. Komrokji, Alan F List, Valeria Santini, Eric Solary, Eric Padron. Comprehensive Inflammatory Cytokine Profiling Identifies IL-8/CXCL8 As Elevated, Associated with Proliferative Features, and Independently Prognostic in Chronic Myelomonocytic Leukemia (CMML). Blood. 2016 Dec 2; 128(22):109-109.
Elisa Dorantes Acosta, Hui Huang, Sara P. Garcia, Elliot Stieglitz, Mignon L. Loh, G C Yuan, Alan B. Cantor. RUNX1 Is a Candidate Transcriptional Effector in Juvenile Myelomonocytic Leukemia. Blood. 2016 Dec 2; 128(22):2699-2699.
Ilaria Iacobucci, Ji Wen, Manja Meggendorfer, Catherine Carmichael, John K Choi, Katherine Masih, Yongjin Li, Debbie Payne, Daisuke Tomizawa, Nobutaka Kiyokawa, Laura J. Janke, Thomas B. Alexander, Stanley Pounds, Lei Shi, Marcus Valentine, Giuseppe Basso, Franco Locatelli, Shirley Kham Kow Yin, Allen Yeoh Eng Juh, Ries E Rhonda, Elliot Stieglitz, Andrew Wei, Ian D Lewis, Richard D'Andrea, Benjamin T. Kile, Jinghui Zhang, Mignon L. Loh, Soheil Meshinchi, Torsten Haferlach, Charles Mullighan. The Genomic Landscape of Childhood and Adult Acute Erythroid Leukemia. Blood. 2016 Dec 2; 128(22):39-39.
Ceremsak JJ, Yu A, Esquivel E, Lissewski C, Zenker M, Loh ML, Stieglitz E. Germline RRAS2 mutations are not associated with Noonan syndrome. J Med Genet. 2016 Nov; 53(11):728.
Stieglitz E, Loh ML. Pediatric MDS: GATA screen the germline. Blood. 2016 Mar 17; 127(11):1377-8.
Stieglitz E, Taylor-Weiner AN, Chang TY, Gelston LC, Wang YD, Mazor T, Esquivel E, Yu A, Seepo S, Olsen SR, Rosenberg M, Archambeault SL, Abusin G, Beckman K, Brown PA, Briones M, Carcamo B, Cooper T, Dahl GV, Emanuel PD, Fluchel MN, Goyal RK, Hayashi RJ, Hitzler J, Hugge C, Liu YL, Messinger YH, Mahoney DH, Monteleone P, Nemecek ER, Roehrs PA, Schore RJ, Stine KC, Takemoto CM, Toretsky JA, Costello JF, Olshen AB, Stewart C, Li Y, Ma J, Gerbing RB, Alonzo TA, Getz G, Gruber TA, Golub TR, Stegmaier K, Loh ML. Corrigendum: The genomic landscape of juvenile myelomonocytic leukemia. Nat Genet. 2016 Jan; 48(1):101.
Stieglitz E, Ward AF, Gerbing RB, Alonzo TA, Arceci RJ, Liu YL, Emanuel PD, Widemann BC, Cheng JW, Jayaprakash N, Balis FM, Castleberry RP, Bunin NJ, Loh ML, Cooper TM. Phase II/III trial of a pre-transplant farnesyl transferase inhibitor in juvenile myelomonocytic leukemia: a report from the Children's Oncology Group. Pediatr Blood Cancer. 2015 Apr; 62(4):629-36.
Elliot Stieglitz, Camille B Troup, Laura C Gelston, Eric D Chow, Kristie B Yu, Jon Akutagawa, Amaro N Taylor-Weiner, Y. Lucy Liu, Peter D Emanuel, Benjamin S Braun, Robert B. Gerbing, Todd A. Alonzo, Mignon L. Loh. Subclonal Mutations in SETBP1 Predict Relapse in Juvenile Myelomonocytic Leukemia. Blood. 2014 Dec 6; 124(21):410-410.
Stieglitz E, Troup CB, Gelston LC, Haliburton J, Chow ED, Yu KB, Akutagawa J, Taylor-Weiner AN, Liu YL, Wang YD, Beckman K, Emanuel PD, Braun BS, Abate A, Gerbing RB, Alonzo TA, Loh ML. Subclonal mutations in SETBP1 confer a poor prognosis in juvenile myelomonocytic leukemia. Blood. 2015 Jan 15; 125(3):516-24.
Stieglitz E, Liu YL, Emanuel PD, Castleberry RP, Cooper TM, Shannon KM, Loh ML. Mutations in GATA2 are rare in juvenile myelomonocytic leukemia. Blood. 2014 Feb 27; 123(9):1426-7.
Stieglitz E, Hsiang MS, Simko JP, Hirose S, Goldsby RE. Pulmonary coccidiomycosis masquerading as refractory metastatic Ewing sarcoma. J Pediatr Hematol Oncol. 2014 Jan; 36(1):e57-60.
Elliot Stieglitz, Y. Lucy Liu, Peter D. Emanuel, Robert P. Castleberry, Todd Michael Cooper, Kevin Shannon, Mignon L. Loh. Mutations In GATA2 Are Rare In Juvenile Myelomonocytic Leukemia. Blood. 2013 Nov 15; 122(21):1526-1526.
Stieglitz E, Loh ML. Genetic predispositions to childhood leukemia. Ther Adv Hematol. 2013 Aug; 4(4):270-90.