Luke Judge, MD, PhD

Dr. Judge is a physician-scientist with extensive experience with mouse and human models of genetic disease. His current research utilizes induced pluripotent stem cells (iPSC) and gene editing to model disease and develop therapies. His primary interests focus on therapeutic gene editing for dominant neurologic disorders. He is funded by the NIH and Charcot-Marie-Tooth Association to evaluate therapeutic gene editing strategies in human and murine models of genetic neuropathy and published the first study of a gene editing approach for axonal neuropathy (Feliciano et al, 2021). Dr. Judge is an Affiliate Investigator in the Gladstone Institute of Data Science and Biotechnology as well as a practicing neonatologist at UCSF Benioff Children’s Hospital. He is an active mentor in both the clinical and laboratory realms and directs the fellowship program in Neonatal-Perinatal medicine.
Education
2019 - Diversity, Equity, and Inclusion Champion Training, University of California
Fellowship, 2014 - Neonatal-Perinatal Medicine, University of California, San Francisco
Residency, 2011 - Pediatrics, University of California, San Francisco
M.D., 2008 - Medicine, University of Washington
Ph.D., 2006 - Molecular and Cellular Biology, University of Washington
B.A., 1997 - Chemistry, Pomona College

  1. Ramadoss GN, Namaganda SJ, Hamilton JR, Sharma R, Chow KG, Macklin BL, Sun M, Liu JC, Fellmann C, Watry HL, Jin J, Perez BS, Sandoval Espinoza CR, Matia MP, Lu SH, Judge LM, Nussenzweig A, Adamson B, Murthy N, Doudna JA, Kampmann M, Conklin BR. Neuronal DNA repair reveals strategies to influence CRISPR editing outcomes. bioRxiv : the preprint server for biology 2024. PMID: 38979269


  2. Van Lent J, Prior R, Pérez Siles G, Cutrupi AN, Kennerson ML, Vangansewinkel T, Wolfs E, Mukherjee-Clavin B, Nevin Z, Judge L, Conklin B, Tyynismaa H, Clark AJ, Bennett DL, Van Den Bosch L, Saporta M, Timmerman V. Advances and challenges in modeling inherited peripheral neuropathies using iPSCs. Experimental & molecular medicine 2024. PMID: 38825644


  3. Perez-Bermejo JA, Judge LM, Jensen CL, Wu K, Watry HL, Truong A, Ho JJ, Carter M, Runyon WV, Kaake RM, Pulido EH, Mandegar MA, Swaney DL, So PL, Krogan NJ, Conklin BR. Functional analysis of a common BAG3 allele associated with protection from heart failure. Nature Cardiovascular Research 2023. PMID: 39195919


  4. Feliciano CM, Wu K, Watry HL, Marley CBE, Ramadoss GN, Ghanim HY, Liu AZ, Zholudeva LV, McDevitt TC, Saporta MA, Conklin BR, Judge LM. Allele-Specific Gene Editing Rescues Pathology in a Human Model of Charcot-Marie-Tooth Disease Type 2E. Frontiers in cell and developmental biology 2021. PMID: 34485306


  5. Vila OF, Chavez M, Ma SP, Yeager K, Zholudeva LV, Colón-Mercado JM, Qu Y, Nash TR, Lai C, Feliciano CM, Carter M, Kamm RD, Judge LM, Conklin BR, Ward ME, McDevitt TC, Vunjak-Novakovic G. Bioengineered optogenetic model of human neuromuscular junction. Biomaterials 2021. PMID: 34403849


  6. Watry HL, Feliciano CM, Gjoni K, Takahashi G, Miyaoka Y, Conklin BR, Judge LM. Rapid, precise quantification of large DNA excisions and inversions by ddPCR. Scientific Reports 2020. PMID: 32913194


  7. Juan A Perez-Bermejo, Luke M Judge, Kenneth Wu, Christina L Jensen, Robyn M Kaake, Danielle Swaney, Po-Lin So, Nevan J Krogan, Bruce R Conklin. Abstract 934: Physical and Genetic Interaction Analyses in Human Pluripotent Stem Cell-Derived Cardiomyocytes to Study Protein Quality Control Disease in the Heart. Circulation research 2019. PMID:


  8. Song M, Yang X, Ren X, Maliskova L, Li B, Jones IR, Wang C, Jacob F, Wu K, Traglia M, Tam TW, Jamieson K, Lu SY, Ming GL, Li Y, Yao J, Weiss LA, Dixon JR, Judge LM, Conklin BR, Song H, Gan L, Shen Y. Mapping cis-regulatory chromatin contacts in neural cells links neuropsychiatric disorder risk variants to target genes. Nature genetics 2019. PMID: 31367015


  9. Wienert B, Wyman SK, Richardson CD, Yeh CD, Akcakaya P, Porritt MJ, Morlock M, Vu JT, Kazane KR, Watry HL, Judge LM, Conklin BR, Maresca M, Corn JE. Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq. Science (New York, N.Y.) 2019. PMID: 31000663


  10. Michael Song, Xiaoyu Yang, Xingjie Ren, Lenka Maliskova, Bingkun Li, Ian Jones, Chao Wang, Fadi Jacob, Kenneth Wu, Michela Traglia, Tsz Wai Tam, Kirsty Jamieson, Si-Yao Lu, Guo-Li Ming, Jun Yao, Lauren A. Weiss, Jesse Dixon, Luke M. Judge, Bruce R. Conklin, Hongjun Song, Li Gan, Yin Shen. cis-Regulatory Chromatin Contacts in Neural Cells Reveal Contributions of Genetic Variants to Complex Neurological Disorders. bioRxiv 2018. PMID:


  11. Beeke Wienert, Stacia K Wyman, Christopher D Richardson, Charles D Yeh, Pinar Akcakaya, Michelle J Porritt, Michaela Morlock, Jonathan T Vu, Katelynn R Kazane, Hannah L Watry, Luke M Judge, Bruce R Conklin, Marcello Maresca, Jacob E Corn. Unbiased detection of CRISPR off-targets in vivo using DISCOVER-Seq. bioRxiv 2018. PMID:


  12. Abu-El-Haija A, Fineman J, Connolly AJ, Murali P, Judge LM, Slavotinek AM. Two patients with FOXF1 mutations with alveolar capillary dysplasia with misalignment of pulmonary veins and other malformations: Two different presentations and outcomes. American journal of medical genetics. Part A 2018. PMID: 30380203


  13. Nelson DM, Lindsay A, Judge LM, Duan D, Chamberlain JS, Lowe DA, Ervasti JM. Variable rescue of microtubule and physiological phenotypes in mdx muscle expressing different miniaturized dystrophins. Human molecular genetics 2018. PMID: 29901725


  14. Nelson DM, Lindsay A, Judge LM, Duan D, Chamberlain JS, Lowe DA, Ervasti JM. Variable rescue of microtubule and physiological phenotypes in mdx muscle expressing different miniaturized dystrophins. Human molecular genetics 2018. PMID: 29618008


  15. Judge LM, Perez-Bermejo JA, Truong A, Ribeiro AJ, Yoo JC, Jensen CL, Mandegar MA, Huebsch N, Kaake RM, So PL, Srivastava D, Pruitt BL, Krogan NJ, Conklin BR. A BAG3 chaperone complex maintains cardiomyocyte function during proteotoxic stress. JCI insight 2017. PMID: 28724793


  16. Huebsch N, Loskill P, Deveshwar N, Spencer CI, Judge LM, Mandegar MA, Fox CB, Mohamed TM, Ma Z, Mathur A, Sheehan AM, Truong A, Saxton M, Yoo J, Srivastava D, Desai TA, So PL, Healy KE, Conklin BR. Miniaturized iPS-Cell-Derived Cardiac Muscles for Physiologically Relevant Drug Response Analyses. Scientific Reports 2016. PMID: 27095412


  17. Mandegar MA, Huebsch N, Frolov EB, Shin E, Truong A, Olvera MP, Chan AH, Miyaoka Y, Holmes K, Spencer CI, Judge LM, Gordon DE, Eskildsen TV, Villalta JE, Horlbeck MA, Gilbert LA, Krogan NJ, Sheikh SP, Weissman JS, Qi LS, So PL, Conklin BR. CRISPR Interference Efficiently Induces Specific and Reversible Gene Silencing in Human iPSCs. Cell stem cell 2016. PMID: 26971820


  18. Huebsch N, Loskill P, Mandegar MA, Marks NC, Sheehan AS, Ma Z, Mathur A, Nguyen TN, Yoo JC, Judge LM, Spencer CI, Chukka AC, Russell CR, So PL, Conklin BR, Healy KE. Automated Video-Based Analysis of Contractility and Calcium Flux in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes Cultured over Different Spatial Scales. Tissue engineering. Part C, Methods 2015. PMID: 25333967


  19. Miyaoka Y, Chan AH, Judge LM, Yoo J, Huang M, Nguyen TD, Lizarraga PP, So PL, Conklin BR. Isolation of single-base genome-edited human iPS cells without antibiotic selection. Nature methods 2014. PMID: 24509632


  20. Judge LM, Arnett AL, Banks GB, Chamberlain JS. Expression of the dystrophin isoform Dp116 preserves functional muscle mass and extends lifespan without preventing dystrophy in severely dystrophic mice. Human molecular genetics 2011. PMID: 21949353


  21. Banks GB, Judge LM, Allen JM, Chamberlain JS. The polyproline site in hinge 2 influences the functional capacity of truncated dystrophins. PLoS genetics 2010. PMID: 20502633


  22. Li D, Bareja A, Judge L, Yue Y, Lai Y, Fairclough R, Davies KE, Chamberlain JS, Duan D. Sarcolemmal nNOS anchoring reveals a qualitative difference between dystrophin and utrophin. Journal of cell science 2010. PMID: 20483958


  23. Lai Y, Thomas GD, Yue Y, Yang HT, Li D, Long C, Judge L, Bostick B, Chamberlain JS, Terjung RL, Duan D. Dystrophins carrying spectrin-like repeats 16 and 17 anchor nNOS to the sarcolemma and enhance exercise performance in a mouse model of muscular dystrophy. The Journal of clinical investigation 2009. PMID: 19229108


  24. Judge LM, Haraguchiln M, Chamberlain JS. Dissecting the signaling and mechanical functions of the dystrophin-glycoprotein complex. Journal of cell science 2006. PMID: 16569668


  25. Judge LM, Chamberlain JS. Gene therapy for Duchenne muscular dystrophy: AAV leads the way. Acta myologica : myopathies and cardiomyopathies : official journal of the Mediterranean Society of Myology 2005. PMID: 16629052


  26. Andersen HS, Olsen OH, Iversen LF, Sørensen AL, Mortensen SB, Christensen MS, Branner S, Hansen TK, Lau JF, Jeppesen L, Moran EJ, Su J, Bakir F, Judge L, Shahbaz M, Collins T, Vo T, Newman MJ, Ripka WC, Møller NP. Discovery and SAR of a novel selective and orally bioavailable nonpeptide classical competitive inhibitor class of protein-tyrosine phosphatase 1B. Journal of medicinal chemistry 2002. PMID: 12238924


  27. Jesper F Lau, Thomas Kruse Hansen, John Paul Kilburn, Karla Frydenvang, Daniel D Holsworth, Yu Ge, Roy T Uyeda, Luke M Judge, Henrik Sune Andersen. A convenient large-scale chiral synthesis of protected 2-substituted 4-oxo-piperidine derivatives. Tetrahedron 2002. PMID:


  28. Niswender CM, Ishihara RW, Judge LM, Zhang C, Shokat KM, McKnight GS. Protein engineering of protein kinase A catalytic subunits results in the acquisition of novel inhibitor sensitivity. The Journal of biological chemistry 2002. PMID: 12034735


  29. Luke M. Judge, Daniel J. O'Leary, Gary Fujii, Craig Skenes, Tom Paquette, Richard T. Proffitt. A hydrazino nicotinamide derivative of cholesterol for radiolabelling liposomes with 99mTc. Journal of Labelled Compounds and Radiopharmaceuticals 1999. PMID: