Laura Hertel, PhD

We study the tropism and pathogenesis of human cytomegalovirus (CMV).
Our current goals are to understand the mechanisms supporting CMV transmission from host to host and CMV spread within infected individuals. We seek to identify the cell types targeted during initial entry, the cellular proteins that mediate infection-induced cell-cell fusion and the viral factors that support or antagonize infection.
Education
Postdoctoral Fellow, - Molecular Virology, Stanford University
Research Associate, - Molecular Virology, Stanford University
B.Sc., - Biological Sciences, University of Torino
Ph.D, - Immunology, University of Torino
Honors and Awards
  • Students’ Council Award of Excellence in teaching in Microbiology and Immunology, The University of Western Ontario, 2006-2009
  • Stanford School of Medicine Dean’s Fellowship, Stanford University, 2000-2001
Websites
Publications
  1. Human Cytomegalovirus Replication and Infection-Induced Syncytia Formation in Labial, Foreskin, and Fetal Lung Fibroblasts.
  2. Genome sequences of human cytomegalovirus strain TB40/E variants propagated in fibroblasts and epithelial cells.
  3. LoReTTA, a user-friendly tool for assembling viral genomes from PacBio sequence data.
  4. Cytomegalovirus Strain TB40/E Restrictions and Adaptations to Growth in ARPE-19 Epithelial Cells.
  5. Genome Sequence of Human Cytomegalovirus Ig-KG-H2, a Variant of Strain KG Propagated in the Presence of Neutralizing Antibodies.
  6. Comparative neutralizing potencies of antibodies suggest conservation as well as mechanistic differences in human cytomegalovirus entry into epithelial and endothelial cells.
  7. Single-Cell Transcriptome Analysis of CD34+ Stem Cell-Derived Myeloid Cells Infected With Human Cytomegalovirus.
  8. Inclusion of Antibodies to Cell Culture Media Preserves the Integrity of Genes Encoding RL13 and the Pentameric Complex Components During Fibroblast Passage of Human Cytomegalovirus.
  9. Activation of Langerhans-Type Dendritic Cells Alters Human Cytomegalovirus Infection and Reactivation in a Stimulus-Dependent Manner.
  10. Dynamics of Human Cytomegalovirus Infection in CD34+ Hematopoietic Cells and Derived Langerhans-Type Dendritic Cells.
  11. Human cytomegalovirus tropism for mucosal myeloid dendritic cells.
  12. Human cytomegalovirus infection of langerhans-type dendritic cells does not require the presence of the gH/gL/UL128-131A complex and is blocked after nuclear deposition of viral genomes in immature cells.
  13. Herpesviruses and intermediate filaments: close encounters with the third type.
  14. Human cytomegalovirus decreases constitutive transcription of MHC class II genes in mature Langerhans cells by reducing CIITA transcript levels.
  15. RASCAL is a new human cytomegalovirus-encoded protein that localizes to the nuclear lamina and in cytoplasmic vesicles at late times postinfection.
  16. Onset of human cytomegalovirus replication in fibroblasts requires the presence of an intact vimentin cytoskeleton.
  17. Human cytomegalovirus US9 protein contains an N-terminal signal sequence and a C-terminal mitochondrial localization domain, and does not alter cellular sensitivity to apoptosis.
  18. Comparisons of CD8+ T cells specific for human immunodeficiency virus, hepatitis C virus, and cytomegalovirus reveal differences in frequency, immunodominance, phenotype, and interleukin-2 responsiveness.
  19. Viral and cell cycle-regulated kinases in cytomegalovirus-induced pseudomitosis and replication.
  20. Human cytomegalovirus alters localization of MHC class II and dendrite morphology in mature Langerhans cells.
  21. Dynamics of recurrent viral infection.
  22. Global analysis of host cell gene expression late during cytomegalovirus infection reveals extensive dysregulation of cell cycle gene expression and induction of Pseudomitosis independent of US28 function.
  23. Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.
  24. The interferon-inducible 204 gene is transcriptionally activated by mouse cytomegalovirus and is required for its replication.
  25. The retinoblastoma protein is an essential mediator that links the interferon-inducible 204 gene to cell-cycle regulation.
  26. The interferon-inducible 204 gene, a member of the Ifi 200 family, is not involved in the antiviral state induction by IFN-alpha, but is required by the mouse cytomegalovirus for its replication.
  27. Demonstration of multiple HPV types in laryngeal premalignant lesions using polymerase chain reaction and immunohistochemistry.
  28. The HMG protein T160 colocalizes with DNA replication foci and is down-regulated during cell differentiation.
  29. In vitro and in vivo expression analysis of the interferon-inducible 203 gene.
  30. Human cytomegalovirus stimulates cellular dihydrofolate reductase activity in quiescent cells.
  31. Overexpression of cellular dihydrofolate reductase abolishes the anticytomegaloviral activity of methotrexate.
  32. Decreased expression of the high-mobility group protein T160 by antisense RNA impairs the growth of mouse fibroblasts.
  33. The high-mobility group protein T160 binds to both linear and cruciform DNA and mediates DNA bending as determined by ring closure.
  34. Suppression of high mobility group protein T160 expression impairs mouse cytomegalovirus replication.
  35. Constitutive expression of the interferon-inducible protein p202 in NIH 3T3 cells affects cell cycle progression.
  36. Defects of glucose-6-phosphate and 6-phosphogluconate dehydrogenases in Neurospora and their pleiotropic effects.
  37. Digitoxin metabolism by rat liver microsomes.
  38. [Review of activities of the Orthopedic Hospital of the Foundation for the care of the Disabled].